protein expression  (R&D Systems)

Journal: Science advances

Article Title: Caspase-1-dependent pyroptosis converts αSMA + CAFs into collagen-III high iCAFs to fuel chemoresistant cancer stem cells.

doi: 10.1126/sciadv.adt8697

Figure Lengend Snippet: Fig. 4. Tumor cell pyroptosis skews CAFs toward iCAFs in a CCR6-dependent mechanism. (A) Representative image illustrating a cytokine protein array (i.e., Proteome Profiler Human XL Cytokine Array Kit, R&D Systems) probed with the supernatant collected from WT and Casp1 KO T24 cancer cells after 48 hours of gemcitabine treat- ment. Red, blue, and green boxes highlighting the individual cytokines or chemokines with the most differential expression (i.e., reduction) in Casp1 KO versus WT Gem- treated supernatant. (B) Bar graph quantifying the intensity of three cytokines differentially released in WT and Casp1 KO supernatant, i.e., CXCL5, CXCL10, and CCL20. (C) ELISA quantification of CXCL5, CXCL10, and CCL20 protein concentration in the supernatants collected from gemcitabine-treated WT and Casp1 KO T24 blad- der cancer cells. (D) Flow cytometry assessing the percentage of αSMAhigh/+ CAFs and αSMA−PDGFβ+ iCAFs treated with CM from Gem-treated T24 cells with anti-CXCR2 neutralizing Ab, (anti-CXCR2 Ab, blocking receptor downstream to CXCL5), anti-CXCL10 neutralizing Ab, anti-CCL20 neutralizing ab, and CCR6i (blocking receptor down- stream to CCL20). (E) Violin dot plot quantifying the relative changes in the percentage of αSMA−PDGFβ+ iCAFs upon CM-Gem ± chemokine or chemokine receptor neutralizing Ab treatments.

Article Snippet: Tumor cell pyroptosis skews CAFs toward iCAFs in a CCR6- dependent mechanism. (A) Representative image illustrating a cytokine protein array (i.e., Proteome Profiler human Xl cytokine Array Kit, R&d Systems) probed with the supernatant collected from Wt and casp1 KO t24 cancer cells after 48 hours of gemcitabine treatment.

Techniques: Protein Array, Quantitative Proteomics, Enzyme-linked Immunosorbent Assay, Protein Concentration, Flow Cytometry, Blocking Assay

Journal: Nutrients

Article Title: Agarwood Oil Nanoemulsion Attenuates Cigarette Smoke-Induced Inflammation and Oxidative Stress Markers in BCi-NS1.1 Airway Epithelial Cells.

doi: 10.3390/nu15041019

Figure Lengend Snippet: Figure 2. Effect of Agarwood-NE on the CSE-induced transcription of the pro-inflammatory cytokine IL-8. BCi-NS1.1 cells were pre-incubated for 1 h in the presence of 25 and 50 µg/mL agarwood-NE, followed by exposure to 5% CSE for 24 h. The mRNA levels of IL-8 were determined via RT-qPCR. Values are expressed as mean ± SEM (n = 4, *: p < 0.05; ****: p < 0.0001).

Article Snippet: The effects of agarwood-NE on cytokine expression levels in CSE-induced BCiNS1.1 cells were assessed using a human cytokine protein array kit (R&D Systems, Minneapolis, MN, USA), as described in a previous study [12].

Techniques: Incubation, Quantitative RT-PCR

Journal: Nutrients

Article Title: Agarwood Oil Nanoemulsion Attenuates Cigarette Smoke-Induced Inflammation and Oxidative Stress Markers in BCi-NS1.1 Airway Epithelial Cells.

doi: 10.3390/nu15041019

Figure Lengend Snippet: Figure 3. Effect of Agarwood-NE on the CSE-induced production of pro-inflammatory mediators in human cytokine protein array. BCi-NS1.1 cells were pre-incubated for 1 h in the presence of 25 and 50 µg/mL agarwood-NE, followed by exposure to 5% CSE for 24 h. The protein levels of IL-1α (A), IL-1β (B), IL-1Ra (C), and GDF-15 (D) were determined via human cytokine protein array.

Article Snippet: The effects of agarwood-NE on cytokine expression levels in CSE-induced BCiNS1.1 cells were assessed using a human cytokine protein array kit (R&D Systems, Minneapolis, MN, USA), as described in a previous study [12].

Techniques: Protein Array, Incubation